The Occurrence and Characterization of Class I, II, and III Integrons Among Carbapenemase-Producing Clinical Strains of Acinetobacter baumannii in Tehran, Iran

Background: Acinetobacter baumannii has emerged as a critical pathogen with high morbidity and mortality in long-term hospitalized patients who stay intensive care units. Carbapenemases integrons are two DNA elements that contribute to the emergence of multidrug-resistant (MDR) A. baumannii. Objectives: The current study aimed at characterization molecular detection class 1, 2, 3 among carbapenemase-producing strains recovered from clinical setting Tehran, Iran. Methods: A total 65 non-replicated were considered this study. Class carbapenemase genes clonal relatedness isolates investigated by PCR assay. Results: prevalence carbapenemases was follows: blaOXA23 (92.31%), blaVIM (69.23%), blaNDM (1.54%). In addition, sequencing confirmed presence gene cassette arrays consisting aacA4-catB8-aadA1 (12/46, 26.09%), aadB-aadA1 (26.09%, 12/46), arr2-cm1A5 (30.43%, 14/46), dfrA1-aadA1 (7.39%, 8/46) 1 integron dfrA1-sat2 (52.94%, 9/17), sat2-aadA1 (47.06%, 8/17) 2 integron. Sequence-based typing both blaOXA-51-like ampC revealed following distribution three different clone types isolates: complex (CC) 10 (46.15%, 30/65), CC2 (40%, 26/65), CC3 (13.85%, 9/65). Statistical analysis showed intI1, blaOXA23, blaVIM, or can significantly increase acquiring MDR phenotypes isolates. Conclusions: High harboring is alarming public health. It seems be served predictive biomarker for setting. However, do not carry these strains.